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Viewing: MLS 4251 : Molecular Diagnostics Laboratory

Last approved: Fri, 02 Oct 2015 08:22:52 GMT

Last edit: Wed, 01 Apr 2015 15:31:37 GMT

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School of Medicine and Health Sciences
Molecular Diagnostics Laboratory
Molecular Dx Lab
Fall 2015
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An introduction to the theory of and laboratory techniques in molecular biology with an emphasis on molecular and serological techniques, including DNA extraction and quantitation, restriction enzyme digestion, polymerase chain reaction, agarose gel electrophoresis, flow cytometry, and ELISA.
Upon successful completion of this course, the student will be able to: • Use appropriate aseptic technique when performing laboratory procedures • Explain the principle of all tests performed in the molecular and serology section of the clinical laboratory • Perform various molecular and serological-based technologies, including: o PCR o Nucleic acid extraction o Electrophoresis o Sequencing o ELISA • Describe the process of nucleic acid extraction, amplification and visualization • Discuss the components needed for nucleic acid electrophoresis • Compare and contrast the various nucleic acid amplification techniques • Differentiate among the various sequencing technologies, including: o Maxam-Gilbert o Chain termination o Next generation • Discuss ethical considerations of molecular test results
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This is a hands-on lab course that will be taught with MLS 4151 Molecular Diagnostics online didactic course in the MLS blended program.
Key: 9792